The study used profilometry (image analysis of skin) to measure the density, depth and volume of wrinkles around the eyes and consisted of treating the area and monitoring the changes over time. In the first split-face, double-blind trial, 49 women applied the Matrixyl cream or a placebo twice daily.

In the active controlled study 16 women compared the effects of cosmetic creams containing Matrixyl or retinol 700 ppm on photoaged skin. This trial also used a split-face, double-blind design and included profilometry, echography, and clinical assessments after two and four months of treatment. At two months, Matrixyl showed greater improvement than the retinol cream without irritation.

In both trials, the company reports, the findings were statistically significant, saying treatment with the MATRIXYL cream produced a:

• 50% decrease in wrinkle depth
• 68% decrease in wrinkle density
• 24% decrease in wrinkle volume.

Matrixyl was also associated with increases in elastin fiber density and thickness and improved regularity of collagen IV, both critical for smooth, supple skin.

Ninety Two (92) women, aged 35 to 55 years, with moderate to severe photodamage applied Matrixyl in a moisturizing base to one side of the face twice daily and placebo to the other side No other skin care products except for a supplied facial cleanser and a broad spectrum UV sunscreen were allowed.

Changes in fine lines and wrinkles were assessed every four weeks by comparisons of high-resolution digital images Efficacy was assessed both by digital image analysis and trained graders, and the study participants were also asked to rate effects on their skin appearance.

Improvement in fine lines and wrinkles on the side where Matrixyl was applied were noted as early as four weeks.

          At the study’s end in 12 weeks:

• Wrinkle length was reduced from 170.4 mm at baseline to 147.7 mm
• Participants' subjective ratings indicated significant benefits for improving age spots, dark circles, and skin firmness.